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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RPA3 Antibody (aa1‑121) LS‑C185337
RPA3 antibody LS-C185337 is an unconjugated rabbit polyclonal antibody to human RPA3. Validated for IHC and WB.
Catalog
Size
Price
LS-C185337-100
100 µl (1 mg/ml)
Unavailable

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Product Description

RPA3 antibody LS-C185337 is an unconjugated rabbit polyclonal antibody to human RPA3. Validated for IHC and WB.
About RPA3
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). P35244 NM_002947 NP_002938.1

RPA3 Antibody, REPA3 Antibody, Replication factor A protein 3 Antibody, Replication protein A3 (14kD) Antibody, Replication protein A3, 14kDa Antibody, RF-A protein 3 Antibody, RP-A p14 Antibody, RPA14 Antibody


Specifications

Target
Human RPA3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RPA3 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 121 of RPA14 (SwissProt P35244). Percent identity by BLAST analysis: Human (100%); Bovine (86%).
Epitope
aa1-121
Specificity
Human RPA3
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
PBS, pH 7.0, 1% BSA, 20% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.
IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.
IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.
IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.
IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/18/2018

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