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Anti-RPA3 Antibody (aa1-121) LS-C185337

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RPA3 Antibodies

Anti-RPA3 Antibody (clone 14.2) LS-C20047
Mouse Monoclonal [clone 14.2] (IgG1) to Human RPA3
Human, Mouse
Western blot, Immunoprecipitation
Unconjugated
Anti-RPA3 Antibody (clone 111) IHC-plus™ LS-B1711
Mouse Monoclonal [clone 111] (IgG1) to Human RPA3
Human, Mouse
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-RPA3 Antibody (N-Terminus, clone 4H9) LS-C53373
Mouse Monoclonal [clone 4H9] (IgG1) to Human RPA3
Human, Mouse
Western blot, Immunoprecipitation
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human RPA3
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human RPA3
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

RPA3 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 121 of RPA14 (SwissProt P35244). Percent identity by BLAST analysis: Human (100%); Bovine (86%).
aa1-121
Human RPA3
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

PBS, pH 7.0, 1% BSA, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About RPA3

P35244 NM_002947 NP_002938.1

RPA3 Antibody, REPA3 Antibody, Replication factor A protein 3 Antibody, Replication protein A3 (14kD) Antibody, Replication protein A3, 14kDa Antibody, RF-A protein 3 Antibody, RP-A p14 Antibody, RPA14 Antibody

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded lung CA patient tumor using RPA3 subunit antibody at 1:100 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: HeLa S3 , B: Hep G2, C: MOLT4. 15% SDS PAGE. RPA3 antibody diluted at 1:1000

Requested From: United States
Date Requested: 12/5/2016

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