Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human RPA2 / RFA2 / RPA34
Human, Mouse, Horse, Pig, Rabbit
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Mouse Monoclonal [clone SPM301] (IgG1,k) to Human RPA2 / RFA2 / RPA34
IHC - Paraffin, Western blot, Immunoprecipitation
Human RPA2 / RFA2 / RPA34
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [SPM301]
IHC - Paraffin (1:50)
Western blot (1 - 2 µg/ml)
Specificity and Use
RPA2 / RFA2 / RPA34 antibody was raised against purified human RPA.
Human RPA. Does not react with rat.
Immunohistochemistry (formalin fixed paraffin embedded): 1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Western Blot: 1-2 ug/ml for 2 hr, RT.
PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months.
RPA2 Antibody, RF-A protein 2 Antibody, RP-A p34 Antibody, RPA34 Antibody, Replication protein A2, 32kDa Antibody, RP-A p32 Antibody, REPA2 Antibody, Replication factor A protein 2 Antibody, Replication protein A2 (32kD) Antibody, RPA32 Antibody
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.