Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Specificity and Use
RP105 / CD180 antibody was raised against m12HRP cell line.
Recognizes human CD180 (RP105).
Suitable for use in Flow Cytometry. Flow Cytometry: Neat-1:10. Use 10 ul of the suggested working dilution to label 1E6 cells in 100ul. Note: CD180 is expressed at the cell surface in association with MS-1. CD180 may also be found free within the cell.
PBS, pH 7.2, 1% BSA, 0.09% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria.