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Anti-ROR1 Antibody LS-C99123

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

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Rabbit Polyclonal to Human ROR1
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human ROR1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

LS-E9178 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99123. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ROR1

Q01973 NM_005012 NP_005003.2

ROR1 Antibody, DJ537F10.1 Antibody, NTRKR1 Antibody, Protein tyrosine kinase t-ror1 Antibody

ROR1 is a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
Western blot of ROR1 (arrow) using rabbit polyclonal ROR1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2) (Origene Technologies).

Western blot

Western blot
Western blot of lysates from K562 cell line, human lung, mouse heart and kidney tissue lysate(from left to right), using ROR1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Flow Cytometry

Flow Cytometry
Flow cytometric of NCI-H460 cells using ROR1 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 4/27/2017

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