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Anti-ROCK2 Antibody (aa511-560) IHC-plus™ LS-B6964

Note: This antibody replaces LS-C139033
Catalog Size Price
LS-B6964-50 50 µg (1 mg/ml) Unavailable

Related Products

46 ROCK2 Antibodies

Most Popular ROCK2 Antibodies

Anti-ROCK2 Antibody (phospho-Tyr256) LS-C60110
Rabbit Polyclonal to Human ROCK2
Human
Western blot, ELISA
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Anti-ROCK-2 pY256 Antibody - Western Blot. Western blot of affinity purified anti-ROCK-2 pY256 antibody shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK-2. A lysate containing a GST null vector also shows no specific staining (data not shown). Primary antibody was used at a 1:1000 dilution. Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.
Anti-ROCK2 Antibody IHC-plus™ LS-B3050
Rabbit Polyclonal (IgG) to Human ROCK2
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IHC, IHC - Paraffin, Western blot
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Anti-ROCK2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3050 dilution 1:100.
Anti-ROCK2 Antibody (clone 1E12) IHC-plus™ LS-B4486
Mouse Monoclonal [clone 1E12] (IgG1,k) to Human ROCK2
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IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
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Anti-ROCK2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4486 concentration 5 ug/ml.
Anti-ROCK2 Antibody (phospho-Tyr722) LS-C155151
Rabbit Polyclonal (IgG) to Human ROCK2
Human, Mouse, Rat, Bovine
ELISA
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Anti-ROCK2 Antibody (N-Terminus, Sepharose) LS-C172065
Rabbit Polyclonal to Human ROCK2
Human, Mouse, Rat
Immunoprecipitation
Sepharose Conjugated

100% Guaranteed
Rabbit Polyclonal to Human ROCK2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
IHC, IHC - Paraffin (applications tested for the base form of this product only)
Unconjugated

Details

Human ROCK2
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Guinea pig, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ROCK2 antibody was raised against synthetic peptide located between aa511-560 of human ROCK2 (O75116, NP_004841). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Horse, Pig (100%); Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (92%); Elephant, Opossum (85%).
LS-E25171 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6964. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa511-560
Human ROCK2.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Sterile deionized water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ROCK2

O75116 NM_004850 NP_004841.2

ROCK2 Antibody, KIAA0619 Antibody, p164 ROCK-2 Antibody, Rho kinase 2 Antibody, RhoA-associated kinase-2 Antibody, ROCK-II Antibody, Rho kinase II Antibody, Rok beta Antibody, Rok2 Antibody, RockII Antibody

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation.

Immunohistochemistry

Anti-ROCK2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6964 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ROCK2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6964 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/21/2018

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