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Anti-ROBO1 Antibody (aa1632-1644) IHC-plus™ LS-B81

Note: This antibody replaces LS-C19082
Catalog Size Price
LS-B81-50 50 µg (1 mg/ml) Unavailable

Related Products

18 ROBO1 Antibodies

Most Popular ROBO1 Antibodies

Anti-ROBO1 Antibody (aa1632-1644) IHC-plus™ LS-B81
Rabbit Polyclonal to Human ROBO1
Human, Mouse, Rat, Dog
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ROBO1 Antibody (aa994-1006) IHC-plus™ LS-B3011
Goat Polyclonal to Human ROBO1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ROBO1 Antibody (aa491-589) LS-C126240
Mouse Monoclonal (IgG) to Human ROBO1
Human
Western blot, ELISA
Unconjugated
Anti-ROBO1 Antibody (clone 1F8) LS-C139227
Mouse Monoclonal [clone 1F8] (IgG2a,k) to Human ROBO1
Human
Western blot, ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image
Anti-ROBO1 Antibody (aa1417-1428) IHC-plus™ LS-B12224
Goat Polyclonal to Mouse ROBO1
Mouse, Bovine, Dog, Horse, Pig
IHC - Paraffin, Western blot, ELISA
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Immunohistochemistry - Paraffin Image

100% Guaranteed
Rabbit Polyclonal to Human ROBO1
Human, Mouse, Rat, Dog
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human ROBO1
Rabbit
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • ELISA (1:30000 - 1:160000)

Specificity and Use

ROBO1 antibody was raised against synthetic peptide from human ROBO1.
aa1632-1644
Amino acids 1632-1644 of Human ROBO-1.
Immunohistochemistry: LS-B81 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B81 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About ROBO1

Q9Y6N7 NM_002941 NP_002932.1

ROBO1 Antibody, Deleted in U twenty twenty Antibody, H-Robo-1 Antibody, Roundabout 1 Antibody, SAX3 Antibody, DUTT1 Antibody, Roundabout homolog 1 Antibody

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration.

Immunohistochemistry

Immunohistochemistry
Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B81 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence - Anti-ROBO-1 Antibody. 1/50 staining mouse lung tissue sections (adult, frozen 100mum whole mount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.

Immunofluorescence

Immunofluorescence
Immunofluorescence - Anti-ROBO-1 Antibody. Staining of ROBO1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining. Image from Lindenmeyer MT et al. Systematic Analysis of a Novel Human Renal Glomerulus-Enriched Gene Expression Dataset. PLoS One. 2010 July 12;5(7): e11545, Fig 5.

Western blot

Western blot
Western Blot - Anti-ROBO-1 Antibody. Western blot of Affinity Purified anti-ROBO-1 antibody shows detection of a band at ~181 kD corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: 
Date Requested: 12/16/2017

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