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Anti-RNF8 Antibody (aa351-400) LS-C80297

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RNF8 Antibodies

Anti-RNF8 Antibody (C-Terminus) LS-C108724
Rabbit Polyclonal to Human RNF8
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Immunofluorescence Image
Anti-RNF8 Antibody (Biotin) LS-C240159
Rabbit Polyclonal (IgG) to Human RNF8
Human
Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Anti-RNF8 Antibody (aa335-364, AP) LS-C240162
Rabbit Polyclonal (IgG) to Human RNF8
Human, Mouse
Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human RNF8
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human RNF8
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

RNF8 antibody was raised against synthetic peptide located between aa351-400 of human RNF8 (O76064, NP_003949). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Bat, Horse, Pig (100%); Galago (92%); Xenopus (91%); Opossum, Turkey (83%).
LS-E14573 - Lyophilized - 100 µg - $145.00
aa351-400
Human RNF8
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
100 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RNF8

O76064 NM_003958 NP_003949.1

RNF8 Antibody, C3HC4-type zinc finger protein Antibody, KIAA0646 Antibody, HRNF8 Antibody, RING finger protein 8 Antibody

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.

Immunohistochemistry

Immunohistochemistry
RNF8 antibody LS-C80297 was used in IHC to stain formalin-fixed, paraffin-embedded human heart. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Jurkat cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/8/2016

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