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Anti-RNF7 Antibody (C-Terminus) LS-C19102

Ordering

Wt. Vol. Conc. Price
- 100 µl 85 mg/ml $405
Inquire for larger quantities

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Most Popular RNF7 Antibodies

Anti-RNF7 Antibody (C-Terminus) LS-C19102
Rabbit Polyclonal to Human RNF7
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-RNF7 Antibody (aa59-71) IHC-plus™ LS-B8759
Goat Polyclonal to Human RNF7
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (3)
Rabbit Polyclonal to Human RNF7
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human RNF7
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)

Specificity and Use

RNF7 antibody was raised against synthetic peptide from human RNF7.
C-Terminus
Amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.
This antibody reacts with human ROC2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins does occur. A 12.6 kD band corresponding to human ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control. Researchers should determine optimal titers for other applications.

Packaging

Sterile filtered antiserum, 0.01% sodium azide.
Store vial at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
For research use only.

Publications (3)

1
Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG, Elledge SJ, Conaway RC, Harper JW, Conaway JW. Science (New York, N.Y.). 1999 284:657-61. [PubMed:10213691]
2
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Molecular cell. 1999 3:535-41. [PubMed:10230407]
3
Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Deshaies RJ, Shevchenko A, Deshaies RJ. Genes & development. 1999 13:1614-26. [PubMed:10385629] [PMC:PMC316801]

About RNF7

Q9UBF6 NM_014245 NP_055060.1

RNF7 Antibody, CKII beta-binding protein 1 Antibody, CKBBP1 Antibody, RING-box protein 2 Antibody, Rbx2 Antibody, Regulator of cullins 2 Antibody, Ring finger protein 7 Antibody, ROC2 Antibody, Zinc RING finger protein SAG Antibody

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F.

Requested From: United States
Date Requested: 12/8/2016

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