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Anti-RNF7 Antibody (aa59-71) IHC-plus™ LS-B8759

Note: This antibody replaces LS-C108944

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RNF7 Antibodies

Anti-RNF7 Antibody (C-Terminus) LS-C19102
Rabbit Polyclonal to Human RNF7
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-RNF7 Antibody (aa59-71) IHC-plus™ LS-B8759
Goat Polyclonal to Human RNF7
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human RNF7
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human RNF7
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA

Specificity and Use

RNF7 antibody was raised against synthetic peptide DACLRCQAENKQE from an internal region of human RNF7 (NP_055060.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Trout, Catfish, Salmon, Sablefish, Stickleback, Pufferfish, Zebrafish (100%); Tick (92%); Eye worm, Seq squirt, Sea anemone (85%).
LS-E27246 - Lyophilized - 100 µg - $145.00
aa59-71
Human RNF7. This antibody is expected to recognize isoform 1 (NP_055060.1).
Western Blot: Approx 11kD band observed in Human Human Heart lysates (calculated MW of 12.7kD according to NP_055060.1). Recommended concentration: 1-3 ug/ml. An additional band of 28kD was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About RNF7

Q9UBF6 NM_014245 NP_055060.1

RNF7 Antibody, CKII beta-binding protein 1 Antibody, CKBBP1 Antibody, RING-box protein 2 Antibody, Rbx2 Antibody, Regulator of cullins 2 Antibody, Ring finger protein 7 Antibody, ROC2 Antibody, Zinc RING finger protein SAG Antibody

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F.

Immunohistochemistry

Immunohistochemistry
Anti-RNF7 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot
RNF7 antibody (1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/10/2016

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