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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RNF6 Antibody (aa578‑627) LS‑C80542
RNF6 antibody LS-C80542 is an unconjugated rabbit polyclonal antibody to RNF6 from human, rat, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C80542-50
50 µg
Unavailable

Popular RNF6 Products

Mouse Intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Mouse Intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Mouse Intestine.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

RNF6 antibody LS-C80542 is an unconjugated rabbit polyclonal antibody to RNF6 from human, rat, bat and other species. Validated for WB.
About RNF6
E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator. Q9Y252 NM_005977 NP_005968.1

RNF6 Antibody, SPG2 Antibody, RING-H2 protein RNF-6 Antibody


Specifications

Target
Human RNF6
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse, Dog, Hamster (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
RNF6 antibody was raised against synthetic peptide located between aa578-627 of human RNF6 (Q9Y252, NP_005968). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Rat, Bat, Rabbit, Horse (100%); Gibbon, Mouse, Hamster, Panda, Dog, Zebra finch (92%); Bovine, Pig, Turkey, Chicken, Platypus, Xenopus (85%); Guinea pig (84%).
Blocking Peptide
LS-E15850 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80542. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa578-627
Specificity
Human RNF6
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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