Human RNF25

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

The antibody also recognizes mouse RNF25 protein. Reactivity against homologues from other sources has not been determined.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:200 - 1:2000)
• Immunoprecipitation
• ELISA (1:10000 - 1:40000)

Immunohistochemistry: LS-B418 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B418 was determined to be 5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96BH1 NCBI: NM_022453 NP_071898.2