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RNF20

ring finger protein 20, E3 ubiquitin protein ligase

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.

Gene Name: ring finger protein 20, E3 ubiquitin protein ligase
Synonyms: RNF20, BRE1, BRE1-A, HBRE1, KAIA2779, RING finger protein 20, BRE1A, Homolog of S. cerevisiae BRE1
Target Sequences: NM_019592 NP_062538.5 Q5VTR2

☰ Filters
Antibodies (30)
Proteins (3)
Over-Expression Lysate (2)
Primary (30)
Recombinant (1)
RNF20 (33)
Human (30)
Mouse (10)
Rat (3)
Bovine (6)
Chimpanzee (6)
Dog (6)
Gibbon (6)
Guinea pig (6)
Hamster (6)
Horse (3)
Monkey (6)
Orangutan (6)
Pig (3)
Rabbit (3)
IHC (6)
IHC-P (4)
WB (25)
ELISA (12)
IF (3)
IP (3)
Peptide-ELISA (2)
rabbit (25)
mouse (5)
Product Group
IHCPlus (2)
IgG (24)
IgG1 (2)
IgG1,k (1)
IgG2b (2)
monoclonal mc (5)
polyclonal pc (25)
1594CT552.128.36 (1)
OTI13A10 (2)
OTI2B9 (2)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (4)
Carrier-free (2)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (16)
aa117-146 (7)
aa108-157 (4)
aa493-754 (4)
aa576-625 (4)
aa125-175 (2)
aa1-50 (1)
No (33)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (2)
293T Cells (1)
HEK 293 Cells (1)
Wheat Germ Extract (1)
RNF20 Antibody - Anti-RNF20 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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RNF20 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
RNF20 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
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RNF20 Rabbit anti-Human Polyclonal (aa125-175) Antibody
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50 µl/$460
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RNF20 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IP, WB
Unconjugated
100 µg/$341
RNF20 Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNF20 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
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RNF20 Mouse anti-Human Monoclonal (aa493-754) (OTI2B9) Antibody
Human
IHC, WB
Unconjugated
100 µl/$434
RNF20 Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNF20 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:150)
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RNF20 Mouse anti-Human Monoclonal (aa493-754) (Carrier-free) (OTI2B9) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
RNF20 Antibody - Immunohistochemistry of paraffin-embedded human ovarian cancer at dilution of 1:100
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RNF20 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
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RNF20 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
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RNF20 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
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RNF20 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
RNF20 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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RNF20 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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RNF20 Rabbit anti-Human Polyclonal (aa117-146) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
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RNF20 Rabbit anti-Human Polyclonal (aa117-146) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
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RNF20 Rabbit anti-Human Polyclonal (aa117-146) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
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RNF20 Rabbit anti-Human Polyclonal (aa117-146) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
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RNF20 Rabbit anti-Human Polyclonal (aa117-146) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
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RNF20 Rabbit anti-Human Polyclonal (aa117-146) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
RNF20 Antibody - Detection of Human RNF20 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RNF20 used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.
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RNF20 Rabbit anti-Human Polyclonal (aa125-175) Antibody
Human
IHC, IHC-P
Unconjugated
2.5 µg/$295; 100 µl/$532
RNF20 Antibody - All lanes: Anti-RNF20 Antibody at 1:2000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: U87-MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 114 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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RNF20 Mouse anti-Human Monoclonal (1594CT552.128.36) Antibody
Human
Peptide-ELISA, WB
Unconjugated
200 µl/$393
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RNF20 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
RNF20 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY RNF20. (Right lane) cDNA for 48 hrs and lysed
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RNF20 Mouse anti-Human Monoclonal (aa493-754) (OTI13A10) Antibody
Human
WB
Unconjugated
100 µl/$434
RNF20 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY RNF20. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF20. (1:500)
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RNF20 Mouse anti-Human Monoclonal (aa493-754) (Carrier-free) (OTI13A10) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
RNF20 Antibody - MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RNF20 Rabbit anti-Human Polyclonal (aa576-625) Antibody
Human
WB
Unconjugated
100 µl/$424
RNF20 Antibody - RNF20 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RNF20 Rabbit anti-Human Polyclonal (aa108-157) Antibody
Human
WB
Unconjugated
100 µl/$424
RNF20 Antibody - RNF20 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RNF20 Rabbit anti-Human Polyclonal (aa108-157) (FITC) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Horse, Orangutan, Gibbon, Chimpanzee, Human, Monkey
WB
FITC Conjugated
100 µl/$469
RNF20 Antibody - RNF20 Antibody western blot of 293 cell line lysates (35 ug/lane). The RNF20 antibody detected the RNF20 protein (arrow).
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RNF20 Rabbit anti-Human Polyclonal (aa117-146) Antibody
Human
WB
Unconjugated
400 µl/$393
Viewing 1-25 of 33 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).