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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RNF20 Antibody (aa108‑157) LS‑C102016
RNF20 antibody LS-C102016 is an unconjugated rabbit polyclonal antibody to RNF20 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102016-50
50 µg
Unavailable

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Product Description

RNF20 antibody LS-C102016 is an unconjugated rabbit polyclonal antibody to RNF20 from human, mouse, rat and other species. Validated for WB.
About RNF20
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. Q5VTR2 NM_019592 NP_062538.5

RNF20 Antibody, BRE1 Antibody, BRE1-A Antibody, HBRE1 Antibody, KAIA2779 Antibody, RING finger protein 20 Antibody, BRE1A Antibody, Homolog of S. cerevisiae BRE1 Antibody


Specifications

Target
Human RNF20
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RNF20 antibody was raised against synthetic peptide located between aa108-157 of human RNF20 (Q5VTR2, NP_062538). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Opossum, Guinea pig (100%); Bat, Pig, Chicken (92%).
Blocking Peptide
LS-E15921 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102016. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa108-157
Specificity
Human RNF20
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF20 antibody LS-C102016 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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