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Anti-RNF2 / RING2 / RING1B Antibody (aa189-201) IHC-plus™ LS-B417

Note: This antibody replaces LS-C19098

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RNF2 / RING2 / RING1B Antibodies

Anti-RNF2 / RING2 / RING1B Antibody (clone 3-3) LS-C179401
Mouse Monoclonal [clone 3-3] (IgG2b) to Mouse RNF2 / RING2 / RING1B
Mouse, Human, Hamster
Western blot, Immunoprecipitation
Unconjugated
Immunoprecipitation Image
Anti-RNF2 / RING2 / RING1B Antibody (aa150-200) IHC-plus™ LS-B12347
Rabbit Polyclonal (IgG) to Human RNF2 / RING2 / RING1B
Human, Mouse, Rat, Bovine, Dog, Chicken, Xenopus
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human RNF2 / RING2 / RING1B
Human, Mouse, Rat, Bovine, Dog, Chicken, Frog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human RNF2 / RING2 / RING1B
Goat
Human, Mouse, Rat, Bovine, Dog, Chicken, Frog (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)

Specificity and Use

RNF2 / RING2 / RING1B antibody was raised against synthetic peptide from human RNF2.
aa189-201
aa 189-201 of human RING1B protein.
Immunohistochemistry: LS-B417 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B417 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About RNF2 / RING2 / RING1B

Q99496 NM_007212 NP_009143.1

RNF2 Antibody, DING Antibody, HIP2-interacting protein 3 Antibody, Protein DinG Antibody, Ring finger protein 2 Antibody, RING finger protein BAP-1 Antibody, BAP-1 Antibody, HIPI3 Antibody, RING finger protein 1B Antibody, RING1B Antibody, RING2 Antibody

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation.

Immunohistochemistry

Immunohistochemistry
Anti-RNF2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B417 concentration 2.5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-RNF2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B417 concentration 2.5 ug/ml.

Western blot

Western blot
Anti-RING1B Antibody - Western Blot. Western blot of Affinity Purified anti-RING1B antibody shows detection of a 38 kD band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.
 

Requested From: United States
Date Requested: 12/10/2016

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