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Anti-RNF174 / MARCH4 Antibody (aa300-350) IHC-plus™ LS-B1796

Note: This antibody replaces LS-C47350

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RNF174 / MARCH4 Antibodies

Anti-RNF174 / MARCH4 Antibody (aa300-350) IHC-plus™ LS-B1796
Rabbit Polyclonal to Human RNF174 / MARCH4
Human, Mouse, Rat, Dog, Horse, Opossum, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RNF174 / MARCH4 Antibody (aa302-331) LS-C158099
Rabbit Polyclonal to Human RNF174 / MARCH4
Human
Western blot
Unconjugated
Western blot Image
Anti-RNF174 / MARCH4 Antibody (aa302-331, Azide-free, HRP) LS-C248765
Rabbit Polyclonal (IgG) to Human RNF174 / MARCH4
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human RNF174 / MARCH4
Human, Mouse, Rat, Dog, Horse, Opossum, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated

Details

Human RNF174 / MARCH4
Rabbit
Human, Mouse, Rat, Dog, Horse, Opossum, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)

Specificity and Use

RNF174 / MARCH4 antibody was raised against a portion of amino acids 300-350of human RNF174.
aa300-350
A portion of amino acids 300-350 of human RNF174.
Immunohistochemistry: LS-B1796 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1796 was determined to be 5 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About RNF174 / MARCH4

Q9P2E8 NP_065865.1

MARCH4 Antibody, RING finger protein 174 Antibody, KIAA1399 Antibody, MARCH-IV Antibody, RNF174 Antibody

E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Immunohistochemistry

Immunohistochemistry
Anti-MARCH4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1796 concentration 5 ug/ml.

Western blot

Western blot
Western blot of human RNF174 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 0.5 ug/ml.

Requested From: United States
Date Requested: 12/5/2016

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