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Anti-RNF146 Antibody (C-Terminus) LS-C80565

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RNF146 Antibodies

Anti-RNF146 Antibody (C-Terminus) LS-C80565
Rabbit Polyclonal (IgG) to Human RNF146
Human, Mouse, Dog, Horse
Western blot
Unconjugated
Western blot Image
Anti-RNF146 Antibody (C-Terminus, Biotin) LS-C445006
Rabbit Polyclonal (IgG) to Human RNF146
Human, Chimpanzee, Orangutan, Mouse, Dog, Horse
Western blot
Biotin Conjugated
Western blot Image
Anti-RNF146 Antibody (C-Terminus, FITC) LS-C445007
Rabbit Polyclonal (IgG) to Human RNF146
Human, Chimpanzee, Orangutan, Mouse, Dog, Horse
Western blot
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human RNF146
Human, Mouse, Dog, Horse
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human RNF146
Rabbit
Human, Mouse, Dog, Horse (tested or 100% immunogen sequence identity)
Monkey, Guinea pig, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

RNF146 antibody was raised against synthetic peptide from C-Terminus of human RNF146 (Q9NTX7-2, NP_112225). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Marmoset, Mouse, Horse (100%); Gibbon, Monkey, Panda, Pig, Guinea pig (92%); Dog (85%); Elephant (82%).
C-Terminus
Human RNF146
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
100 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RNF146

Q9NTX7 NM_030963 NP_112225.2

RNF146 Antibody, DJ351K20.1 Antibody, Iduna Antibody, Ring finger protein 146 Antibody, Dactylidin Antibody, RP3-351K20.1 Antibody

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex.

Western blot

Western blot
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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