LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
RNF144B Antibody (clone 11F4) LS‑C114786
RNF144B antibody LS-C114786 is an unconjugated mouse monoclonal antibody to human RNF144B. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C114786-100
100 µl
Unavailable

Popular RNF144B Products

Anti-RNF144B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Ovary tissue using anti-RNF144B mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-RNF144B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-RNF144B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4550 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Western blot of RNF144b antibody
Species: Human, Mouse
Applications: Western blot, ELISA

Product Description

RNF144B antibody LS-C114786 is an unconjugated mouse monoclonal antibody to human RNF144B. Validated for Flow, IF and WB.
About RNF144B
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. Q7Z419 Q7Z419

RNF144B Antibody, BA528A10.3 Antibody, IBRDC2 Antibody, p53RFP Antibody, IBR domain containing 2 Antibody, PIR2 Antibody, Ring finger protein 144B Antibody


Specifications

Target
Human RNF144B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [11F4]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
RNF144B antibody was raised against human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.
Specificity
Human RNF144B
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunofluorescence

Anti-RNF144B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.
Anti-RNF144B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-RNF144B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.
Anti-RNF144B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-RNF144B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.
Anti-RNF144B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-RNF144B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.
Anti-RNF144B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B.

Immunofluorescence

Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.
Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-RNF144B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY RNF144B (Red) or empty vector control plasmid (Blue) were immunostained with anti-RNF144B mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/22/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number