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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RNF14 / ARA54 Antibody (N‑Terminus) LS‑C134969
ARA54 antibody LS-C134969 is an unconjugated rabbit polyclonal antibody to ARA54 (RNF14) from human, rat, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C134969-50
50 µg
Unavailable

Popular RNF14 / ARA54 Products

RNF14 antibody LS-C31661 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bovine, Dog, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Immunohistochemistry of Human liver cancer using RNF14 Polyclonal Antibody at dilution of 1:20.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
RNF14 antibody LS-C31661 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Monkey, Rat, Bovine, Dog, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

ARA54 antibody LS-C134969 is an unconjugated rabbit polyclonal antibody to ARA54 (RNF14) from human, rat, bat and other species. Validated for WB.
About RNF14 / ARA54
RNF14 / ARA54 contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. Q9UBS8 NM_004290 NP_004281.1

RNF14 Antibody, HRIHFB2038 Antibody, HFB30 Antibody, Triad2 protein Antibody, ARA54 Antibody, Ring finger protein 14 Antibody, TRIAD2 Antibody


Specifications

Target
Rat RNF14 / ARA54
Host
Rabbit
Reactivity
Rat, Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RNF14 / ARA54 antibody was raised against synthetic peptide from N-Terminus of rat Rnf14 (Q3ZAU6, NP_001030167). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Mouse (92%); Opossum (85%); Platypus (84%).
Blocking Peptide
LS-E11922 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C134969. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Rat RNF14
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RNF14 antibody LS-C134969 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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