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RNF114 / ZNF313 Antibody (clone 4G3‑1A10) LS‑C105157 Best Seller
ZNF313 antibody LS-C105157 is an unconjugated mouse monoclonal antibody to human ZNF313 (RNF114). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C105157-100
100 µg
Unavailable

Popular RNF114 / ZNF313 Products

RNF114 / ZNF313 antibody LS-C116960 Western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit
Applications: Western blot
Antibody
Species: Human
Applications: Western blot
Anti-RNF114 / ZNF313 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10832 concentration 7.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

ZNF313 antibody LS-C105157 is an unconjugated mouse monoclonal antibody to human ZNF313 (RNF114). Validated for ELISA, IHC and WB.
About RNF114 / ZNF313
E3 ubiquitin-protein ligase promoting the ubiquitination and degradation of the CDK inhibitor CDKN1A and probably also CDKN1B and CDKN1C. These activities stimulate cell cycle's G1-to-S phase transition and suppress cellular senescence. May play a role in spermatogenesis. Q9Y508 NM_018683 NP_061153.1

RNF114 Antibody, PSORS12 Antibody, Ring finger protein 114 Antibody, Zinc finger protein 313 Antibody, ZNF228 Antibody, ZNF313 Antibody, Zinc finger protein 228 Antibody


Specifications

Target
Human RNF114 / ZNF313
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [4G3-1A10]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RNF114 / ZNF313 antibody was raised against zNF313 (AAH13695, 1 a.a. ~ 229 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control HepG2. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human lateral ventricle wall. Sandwich ELISA (Recombinant protein).
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).
Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).

Western blot

Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.
Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.

Western blot

ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.
ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).
Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).

Western blot

Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.
Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.

Western blot

ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.
ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).
Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).

Western blot

Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.
Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.

Western blot

ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.
ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).
Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall (antibody concentration 3 ug/ml).

Western blot

Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.
Western blot of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.

Western blot

ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.
ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western blot of ZNF313 expression in HepG2.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ZNF313 is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/18/2018

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