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Anti-RMA1 / RNF5 Antibody (N-Terminus) LS-C102756

Catalog	Size	Price
LS-C102756-50	50 µg	Unavailable

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Anti-RMA1 / RNF5 Antibody LS-C676411

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Anti-RMA1 / RNF5 Antibody LS-C676416

Antibody:	Rabbit Polyclonal (IgG) to Human RMA1 / RNF5
Reactivity:	Human
Application:	IHC, Western blot, ELISA
Format:	Unconjugated

Antibody:	Rabbit Polyclonal (IgG) to Human RMA1 / RNF5
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

RNF5 antibody LS-C102756 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human RMA1 / RNF5
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)
Predicted:	Guinea pig, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

RMA1 / RNF5 antibody was raised against synthetic peptide from N-Terminus of human RNF5 (Q99942, NP_008844). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Pig (100%); Hamster, Horse, Guinea pig (92%); Rabbit (91%); Bat (85%); Opossum (84%).

Blocking Peptide:

LS-E21292 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102756. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

N-Terminus

Specificity:

Human RNF5

Usage:

Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About RMA1 / RNF5

Uniprot: Q99942 NCBI: NM_006913 NP_008844.1

Synonyms: RNF5 Antibody, G16 Antibody, HsRma1 Antibody, NG2 Antibody, RING5 Antibody, Protein G16 Antibody, Ram1 homolog Antibody, RING finger protein 5 Antibody, RMA1 Antibody

Description: Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin.

(More About RMA1 / RNF5)

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Western blot

RNF5 antibody LS-C102756 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/23/2018

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