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Human RMA1 / RNF5 | |
Rabbit | |
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity) | |
Guinea pig, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity) | |
IgG Polyclonal | |
Unconjugated. Also available conjugated with FITC, HRP, Biotin. | |
Immunoaffinity purified | |
Unmodified |
RMA1 / RNF5 antibody was raised against synthetic peptide from N-Terminus of human RNF5 (Q99942, NP_008844). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Pig (100%); Hamster, Horse, Guinea pig (92%); Rabbit (91%); Bat (85%); Opossum (84%). | ||
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N-Terminus | ||
Human RNF5 | ||
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500. |
Lyophilized from PBS with 2% sucrose | |
Distilled water | |
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles. | |
For research use only. |
RNF5 Antibody, G16 Antibody, HsRma1 Antibody, NG2 Antibody, RING5 Antibody, Protein G16 Antibody, Ram1 homolog Antibody, RING finger protein 5 Antibody, RMA1 Antibody
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin.
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