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RMA1 / RNF5 Antibody (N‑Terminus) LS‑C102756
RNF5 antibody LS-C102756 is an unconjugated rabbit polyclonal antibody to RNF5 (RMA1) from human, mouse, rat and other species. Validated for WB.
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Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Product Description
RNF5 antibody LS-C102756 is an unconjugated rabbit polyclonal antibody to RNF5 (RMA1) from human, mouse, rat and other species. Validated for WB.
About RMA1 / RNF5
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin.
Q99942
NM_006913
NP_008844.1
RNF5 Antibody, G16 Antibody, HsRma1 Antibody, NG2 Antibody, RING5 Antibody, Protein G16 Antibody, Ram1 homolog Antibody, RING finger protein 5 Antibody, RMA1 Antibody
Specifications
Target
Human RMA1 / RNF5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
(tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Hamster, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
RMA1 / RNF5 antibody was raised against synthetic peptide from N-Terminus of human RNF5 (Q99942, NP_008844). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Pig (100%); Hamster, Horse, Guinea pig (92%); Rabbit (91%); Bat (85%); Opossum (84%).
Blocking Peptide
LS-E21292 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102756. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C102756. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human RNF5
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
RNF5 antibody LS-C102756 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
RNF5 antibody LS-C102756 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
RNF5 antibody LS-C102756 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
RNF5 antibody LS-C102756 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
