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Anti-Rituximab Antibody (clone MB2 A4) LS-C188252

Ordering

Wt. Vol. Conc. Price
200 µg - 1 mg/ml $1,060
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Anti-Rituximab Antibody (clone MB2 A4) LS-C188252
Rat Monoclonal [clone MB2 A4] (IgG2a)
Flow Cytometry, ELISA
Unconjugated
Enzyme-Linked Immunosorbent Assay Image

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Rat Monoclonal [clone MB2 A4] (IgG2a)
Flow Cytometry, ELISA
Unconjugated

Details

Rituximab
Rat
IgG2a Monoclonal [MB2 A4]
Unconjugated
Protein G purified
Unmodified

Applications

  • Flow Cytometry (50 µg/ml)
  • ELISA (5 µg/ml)

Specificity and Use

Rituximab antibody was raised against f(ab)2 fragment of Rituximab.
Recognizes the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. The antibody does not recognize other CD20 antibodies. The antibody has been used in ELISA assays to monitor the levels of Rituximab in patient serum following therapy. Has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonized cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab.
ELISA: Use as coating antibody.

Packaging

Tris buffered saline, 0.09% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Rituximab


Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection of Rituximab spiked into 10% human serum by sandwich ELISA. Human anti-Rituximab (HCA186) was used as the coating antibody and detection was performed by adding HRP conjugated anti-Rituximab antibody. Data are shown as the mean of three independent measurements
 

Requested From: United States
Date Requested: 12/5/2016

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