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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RIP4 / ANKRD3 Antibody (aa802‑832) LS‑C97184 Best Seller
ANKRD3 antibody LS-C97184 is an unconjugated rabbit polyclonal antibody to ANKRD3 (RIP4) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C97184-400
400 µl
Unavailable

Popular RIP4 / ANKRD3 Products

Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Western blot analysis using RIPK4 mAb against HEK293 (1) and RIPK4 (AA: 675-784)-hIgGFc transfected HEK293 (2) cell lysate.
Species: Human
Applications: Western blot, Flow Cytometry, ELISA

Product Description

ANKRD3 antibody LS-C97184 is an unconjugated rabbit polyclonal antibody to ANKRD3 (RIP4) from human and mouse. Validated for IHC and WB.
About RIP4 / ANKRD3
ANKRD3 belongs to the RIP subfamily of kinases. ANKRD3 encodes a deduced 784-amino acid protein with a calculated molecular mass of 86 kD. The protein contains 12 subdomains in the N-terminal region that are highly conserved in all protein kinases, 10 ankyrin-like repeats, and a potential nuclear localization signal. ANKRD3 shares 49% sequence similarity with DAP, but lacks the death domain and the calmodulin-binding domain. P57078 NM_020639 NP_065690.2

RIPK4 Antibody, ANKK2 Antibody, Ankyrin repeat domain 3 Antibody, ANKRD3 Antibody, DIK Antibody, NKRD3 Antibody, RIP4 Antibody, PKK Antibody, PPS2 Antibody


Specifications

Target
Human RIP4 / ANKRD3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9279 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97184. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa802-832
Specificity
This RIPK4 (ANKRD3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 802-832 amino acids from the C-terminal region of human RIPK4 (ANKRD3).
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).
ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).

Western blot

Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.
Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).
ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).

Western blot

Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.
Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).
ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).

Western blot

Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.
Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).
ANKRD3 Antibody (Q817) western blot of A549 cell line lysates (35 ug/lane). The ANKRD3 antibody detected the ANKRD3 protein (arrow).

Western blot

Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.
Western blot of anti-ANKRD3-Q817 antibody in mouse kidney tissue lysate (35 ug/lane). ANKRD3-Q817(arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/24/2018

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