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Anti-RING1 Antibody (aa79-263, clone 8C12F4) LS-C191852

Catalog Size Price
LS-C191852-100 100 µg (1 mg/ml) Unavailable

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Immunofluorescence of A431 cells using Ring1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

100% Guaranteed
Mouse Monoclonal [clone 8C12F4] (IgG1) to Human RING1
Human
Flow Cytometry, ELISA
Unconjugated

Details

Human RING1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [8C12F4]
Unconjugated
Purified
Unmodified

Applications

  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

RING1 antibody was raised against purified recombinant fragment of human Ring1 (AA: 79-263) expressed in E. Coli.
aa79-263
Human

Packaging

Purified antibody in PBS containing 0.05% sodium azide
Short term: 4°C; Long term: -20°C.
For research use only.

About RING1

Q06587 NM_002931 NP_002922.2

RING1 Antibody, Polycomb complex protein RING1 Antibody, Ring finger protein 1 Antibody, RING1A Antibody, RNF1 Antibody

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals.

Immunohistochemistry

IHC of paraffin-embedded cervical cancer tissues using Ring1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded cervical cancer tissues using Ring1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded rectum cancer tissues using Ring1 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded rectum cancer tissues using Ring1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot using Ring1 mouse monoclonal antibody against MOLT-4 (1), LNCaP (2), HeLa (3), HEK-293 (4) and Jurkat (5) cell lysate.
Western blot using Ring1 mouse monoclonal antibody against MOLT-4 (1), LNCaP (2), HeLa (3), HEK-293 (4) and Jurkat (5) cell lysate.

Western blot

Western blot using Ring1 monoclonal antibody against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)
Western blot using Ring1 monoclonal antibody against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)

Western blot

Western blot using Ring1 monoclonal antibody against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using Ring1 monoclonal antibody against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: 
Date Requested: 5/27/2018

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