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Anti-RING1 Antibody (aa79-263, clone 8C12F4) LS-C191852

Catalog Size Price
LS-C191852-100 100 µg (1 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 8C12F4] (IgG1) to Human RING1
Human
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human RING1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [8C12F4]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:10000)

Specificity and Use

RING1 antibody was raised against purified recombinant fragment of human Ring1 (AA: 79-263)
aa79-263
Human RING1

Packaging

PBS, 0.05% sodium azide, 0.05% protein stabilizer
Long term: -20°C; Short term: +4°C
For research use only.

About RING1

Q06587 NM_002931 NP_002922.2

RING1 Antibody, Polycomb complex protein RING1 Antibody, Ring finger protein 1 Antibody, RING1A Antibody, RNF1 Antibody

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded cervical cancer tissues using Ring1 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded rectum cancer tissues using Ring1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot
Western blot using Ring1 mouse monoclonal antibody against MOLT-4 (1), LNCaP (2), HeLa (3), HEK-293 (4) and Jurkat (5) cell lysate.

Western blot

Western blot
Western blot using Ring1 monoclonal antibody against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)

Western blot

Western blot
Western blot using Ring1 monoclonal antibody against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: 
Date Requested: 8/18/2017

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