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Anti-RING1 Antibody (aa252-301) LS-C109759

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

Most Popular RING1 Antibodies

Anti-RING1 Antibody (clone 1D5) LS-C139296
Mouse Monoclonal [clone 1D5] (IgG2b,k) to Human RING1
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-RING1 Antibody (clone 2B3) LS-C139300
Mouse Monoclonal [clone 2B3] (IgG2b,k) to Human RING1
Human, Rat
Western blot, ELISA
Unconjugated
Western blot Image
Anti-RING1 Antibody (clone 4E8) LS-C139374
Mouse Monoclonal [clone 4E8] (IgG2b,k) to Human RING1
Human
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human RING1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human RING1
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

RING1 antibody was raised against synthetic peptide located between aa252-301 of human RING1 (Q06587, NP_002922). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%).
LS-E11716 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109759. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa252-301
Human RING1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About RING1

Q06587 NM_002931 NP_002922.2

RING1 Antibody, Polycomb complex protein RING1 Antibody, Ring finger protein 1 Antibody, RING1A Antibody, RNF1 Antibody

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals.

Western blot

Western blot
RING1 antibody LS-C109759 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/28/2017

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