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Anti-RIF1 Antibody (aa2406-2419) IHC-plus™ LS-B1455

Note: This antibody replaces LS-C18976


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular RIF1 Antibodies

Anti-RIF1 Antibody (aa2406-2419) IHC-plus™ LS-B1455
Rabbit Polyclonal to Mouse RIF1
Mouse, Human
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-RIF1 Antibody (C-Terminus) LS-C93308
Rabbit Polyclonal to Human RIF1
Anti-RIF1 Antibody (aa2076-2089) IHC-plus™ LS-B7340
Goat Polyclonal to Human RIF1
IHC - Paraffin, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse RIF1
Mouse, Human
IHC - Paraffin, Western blot, ELISA


Mouse RIF1
Mouse, Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:40000)

Specificity and Use

RIF1 antibody was raised against synthetic peptide from mouse RIF1.
Immunohistochemistry: LS-B1455 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1455 was determined to be 5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About RIF1

Q5UIP0 U79263 NP_060621.3

RIF1 Antibody

Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.


Anti-RIF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1455 concentration 5 ug/ml.


Anti-RIF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1455 concentration 5 ug/ml.

Western blot

Western blot
Anti-Rif1 Antibody - Western Blot. Western blot of Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kD corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/24/2016

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