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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RICTOR Antibody (clone 1F3) IHC‑plus™ LS‑B6357
Note: This antibody replaces LS-C133849
RICTOR antibody LS-B6357 is an unconjugated mouse monoclonal antibody to human RICTOR. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6357-50
50 µg (0.42 mg/ml)
Unavailable

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Product Description

RICTOR antibody LS-B6357 is an unconjugated mouse monoclonal antibody to human RICTOR. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About RICTOR
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. Q6R327 NM_152756 NP_689969.2

RICTOR Antibody, AVO3 homolog Antibody, HAVO3 Antibody, MAVO3 Antibody, PIA Antibody, KIAA1999 Antibody, TORC2-specific protein AVO3 Antibody, AVO3 Antibody, Pianissimo Antibody


Specifications

Target
Human RICTOR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [1F3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RICTOR antibody was raised against rICTOR (NP_689969, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human RICTOR
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4. Sourced in Ascites.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.
Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.

Western blot

RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.
RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.

Western blot

Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.
Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.

Immunoprecipitation

Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.
Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.

Western blot

RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.
RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.

Western blot

Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.
Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.

Immunoprecipitation

Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.
Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.

Western blot

RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.
RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.

Western blot

Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.
Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.

Immunoprecipitation

Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.
Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.

Western blot

RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.
RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.

Western blot

Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.
Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.

Immunoprecipitation

Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/18/2018

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