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Anti-RICTOR Antibody (clone 1F3) IHC-plus™ LS-B6357

Note: This antibody replaces LS-C133849
Catalog Size Price
LS-B6357-50 50 µg (0.42 mg/ml) Unavailable

Related Products

53 RICTOR Antibodies

Most Popular RICTOR Antibodies

Anti-RICTOR Antibody (aa1-50) LS-C19387
Rabbit Polyclonal to Human RICTOR
Human
Western blot
Unconjugated
Western blot Image
Anti-RICTOR Antibody LS-C131923
Mouse Monoclonal (IgG2a,k) to Human RICTOR
Human, Mouse, Rat
Western blot, Immunoprecipitation
Unconjugated
Anti-RICTOR Antibody (clone 1F3) IHC-plus™ LS-B6357
Mouse Monoclonal [clone 1F3] (IgG2b,k) to Human RICTOR
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RICTOR Antibody (C-Terminus) IHC-plus™ LS-B12202
Rabbit Polyclonal (IgG) to Human RICTOR
Human, Mouse, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry - Paraffin Image
Anti-RICTOR Antibody (clone 7B3) LS-C359276
Mouse Monoclonal [clone 7B3] (IgG1) to Human RICTOR
Human, Mouse, Primate
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Mouse Monoclonal [clone 1F3] (IgG2b,k) to Human RICTOR
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human RICTOR
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [1F3]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA

Specificity and Use

RICTOR antibody was raised against rICTOR (NP_689969, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human RICTOR
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.

Packaging

PBS, pH 7.4. Sourced in Ascites.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About RICTOR

Q6R327 NM_152756 NP_689969.2

RICTOR Antibody, AVO3 homolog Antibody, HAVO3 Antibody, MAVO3 Antibody, PIA Antibody, KIAA1999 Antibody, TORC2-specific protein AVO3 Antibody, AVO3 Antibody, Pianissimo Antibody

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin.

Immunohistochemistry

Immunohistochemistry
Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6357 concentration 5 ug/ml.

Western blot

Western blot
RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.

Western blot

Western blot
Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.

Requested From: 
Date Requested: 6/27/2017

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