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Rhodopsin / RHO Antibody (N‑Terminus) LS‑C17693
RHO antibody LS-C17693 is an unconjugated mouse monoclonal antibody to rat RHO (Rhodopsin). Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C17693-125
125 µg (0.5 mg/ml)
$575

Popular Rhodopsin / RHO Products

Anti-RHO / Rhodopsin antibody LS-A1052 IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey, Mouse, Rat, Pig, Rabbit
Applications: IHC, IHC - Paraffin
Pig retinal section stained with Rhodopsin / RHO antibody (green) and counterstained with rabbit polyclonal antibody to neurofilament RPCA-NF-M (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-Rhodopsin / RHO antibody IHC staining of human retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10408 concentration 10 ug/ml.
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Confocal image of mouse retina labeled for Rhodopsin using Rhodopsin / RHO antibody antibody and counterstained with the nuclear stain DAPI (blue). Labeling (Green) is primarily restricted to the photoreceptor outer segment (OS). Other layers of the retina: IS- inner segment; ONL - outer nuclear layer; OPL - outer plexiform layer; OPL - outer plexiform layer; INL - inner nuclear layer; IPL - inner plexiform layer; GCL - ganglion cell layer.
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

RHO antibody LS-C17693 is an unconjugated mouse monoclonal antibody to rat RHO (Rhodopsin). Validated for IHC and WB.

Specifications

Target
Rat Rhodopsin / RHO
Synonyms
RHO, CSNBAD1, OPN2, Rhodopsin, RP4, Opsin 2, rod pigment, Opsin-2
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC (1:25 - 1:50)
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Rhodopsin / RHO antibody was raised against rat Rhodopsin.
Epitope
N-Terminus
Specificity
Recognizes rat Rhodopsin, a 39KD glycoprotein. Epitope sequence is in the first 10 aa at N-terminus (GTEGPNFYV). This antibody specifically labels rods with the axons and synaptic pedicles of the rods being labeled.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Also suitable for use in studies of photoreceptor cells. Immunohistochemistry: 1:25.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About Rhodopsin / RHO
Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. P08100 NM_000539 NP_000530.1


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Requested From: United States
Date Requested: 10/16/2018

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