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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Rhodanese / TST Antibody (aa1‑270) LS‑C185776
TST antibody LS-C185776 is an unconjugated rabbit polyclonal antibody to human TST (Rhodanese). Validated for IHC and WB.
Catalog
Size
Price
LS-C185776-100
100 µl (0.39 mg/ml)
Unavailable

Popular Rhodanese / TST Products

Anti-TST antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4655 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Antibody staining TST in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

TST antibody LS-C185776 is an unconjugated rabbit polyclonal antibody to human TST (Rhodanese). Validated for IHC and WB.
About Rhodanese / TST
Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA. Q16762 NM_003312 NP_003303.2

TST Antibody, Rhodanese Antibody, Thiosulfate sulfurtransferase Antibody, RDS Antibody


Specifications

Target
Human Rhodanese / TST
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Rhodanese / TST antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 270 of TST (SwissProt Q16762).
Epitope
aa1-270
Specificity
Human Rhodanese / TST
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.
IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.

Western blot

TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000
TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.
IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.

Western blot

TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000
TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.
IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.

Western blot

TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000
TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.
IHC of paraffin-embedded MCF7 xenograft using TST antibody at 1:250 dilution.

Western blot

TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000
TST antibody detects TST protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 12 % SDS-PAGE. TST antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: Mouse liver. 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. Rhodanese / TST antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/18/2018

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