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Anti-Rho Kinase / ROCK1 Antibody (aa1114-1126, clone 154C1465) LS-C511

Note: This antibody replaces LS-C93367

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal [clone 154C1465] (IgG) to Human Rho Kinase / ROCK1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse
Western blot
Unconjugated

Details

Human Rho Kinase / ROCK1
Mouse
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
IgG Monoclonal [154C1465]
Protein G purified

Applications

Western blot (1 - 2 µg/ml)
IHC - Paraffin

Specificity and Use

Rho Kinase / ROCK1 antibody was raised against this antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to residues 1114-1126 GNLPESRIEGWLS of human ROCK-1. Immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Dog, Bat, Horse, Opossum, Platypus (100%); Rabbit (92%); Chicken (85%).
aa1114-1126
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Rho Kinase / ROCK1

Q13464 NM_005406 NP_005397.1

ROCK1 Antibody, p160 Rho-associated kinase Antibody, p160ROCK Antibody, p160 ROCK-1 Antibody, p160-ROCK Antibody, p160RhoK Antibody, Rho-associated kinase Antibody, RockI Antibody, Rok Antibody, Rok alpha Antibody, Rho-alpha kinase Antibody, p160 rho-kinase Antibody, Rho kinase Antibody, ROCK-I Antibody, Rokalpha Antibody

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing.

Western blot

Western blot
Detection of cleaved ROCK-1 in apoptotic Jurkat cell lysates (10 µg/lane). Lane 1. Jurkat cells were treated with camptothecin. Lane 2. Jurkat cells were treated with anti-Fas antibody (10 ng/ml for 2 hrs). antibody was used at a concentration of 1 ug/ml.
 

Requested From: United States
Date Requested: 9/26/2016

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