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Anti-RHEB Antibody IHC-plus™ LS-B543

Note: This antibody replaces LS-C477, LS-C43139, LS-B7288

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RHEB Antibodies

Anti-RHEB Antibody IHC-plus™ LS-B543
Rabbit Polyclonal to Human RHEB
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RHEB Antibody (aa107-156, Biotin) LS-C462151
Rabbit Polyclonal (IgG) to Human RHEB
Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken
IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human RHEB
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human RHEB
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

RHEB antibody was raised against synthetic peptide from human RHEB.
LS-E30137 - Liquid - 50 µg - $145.00
15 amino acid peptide from the middle region of human Rheb
Immunohistochemistry: LS-B543 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B543 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

Publications (1)

1
Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptuses. Gao H, Wu G, Spencer TE, Johnson GA, Bazer FW. Biology of reproduction. 2009 81:87-100. (IHC-P; Sheep) [PubMed:19299312]

About RHEB

Q15382 NM_005614 NP_005605.1

RHEB Antibody, RHEB2 Antibody, GTP-binding protein Rheb Antibody, Ras homolog enriched in brain Antibody

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway.

Immunohistochemistry

Immunohistochemistry
Anti-RHEB antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B543 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of Rheb in Mouse Brain cells with Rheb antibody at 20 ug/ml.

Western blot

Western blot
Western blot of Rheb in mouse brain cell lysate with Rheb antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
 

Requested From: United States
Date Requested: 12/6/2016

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