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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RGS5 Antibody (clone 4E12) LS‑C198574
RGS5 antibody LS-C198574 is an unconjugated mouse monoclonal antibody to human RGS5. Validated for ELISA and WB.
Catalog
Size
Price
LS-C198574-100
100 µg
Unavailable

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Product Description

RGS5 antibody LS-C198574 is an unconjugated mouse monoclonal antibody to human RGS5. Validated for ELISA and WB.
About RGS5
RGS5 is a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. O15539 NM_003617 NP_003608.1

RGS5 Antibody, MST092 Antibody, MSTP032 Antibody, MSTP106 Antibody, MST106 Antibody, MST129 Antibody, MSTP092 Antibody, MSTP129 Antibody


Specifications

Target
Human RGS5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [4E12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
RGS5 antibody was raised against rGS5 (NM_003617, aa94-181 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human RGS5
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of RGS5 expression in transfected 293T cell line by RGS5 monoclonal antibody (M01), clone 4E12.Lane 1: RGS5 transfected lysate (Predicted MW: 20.9 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged RGS5 is approximately 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/17/2018

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