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RFP / Red Fluorescent Protein

Red fluorescent protein (RFP) was originally isolated from Discosoma coral as a tetramer referred to as dsRed. Several synthetic variants of the protein have been created, including monomers. It is commonly used to examine intercellular processes such as tracking gene expression and tagging other proteins.

RFP / Red Fluorescent Protein Target Details

Target Name: RFP / Red Fluorescent Protein

Publications (2)

1
Hematopoietic microRNA-126 protects against renal ischemia/reperfusion injury by promoting vascular integrity. Bijkerk R, van Solingen C, de Boer HC, van der Pol P, Khairoun M, de Bruin RG, van Oeveren-Rietdijk AM, Lievers E, Schlagwein N, van Gijlswijk DJ, Roeten MK, Neshati Z, de Vries AA, Rodijk M, Pike-Overzet K, van den Berg YW, van der Veer EP, Versteeg HH, Reinders ME, Staal FJ, van Kooten C, Rabelink TJ, van Zonneveld AJ. Journal of the American Society of Nephrology : JASN. 2014 25:1710-22. (ELISA; Discosoma) [Full Text Article] [PubMed:24610930] [PMC:PMC4116053]
2
Increased cell-intrinsic excitability induces synaptic changes in new neurons in the adult dentate gyrus that require Npas4. Sim S, Antolin S, Lin CW, Lin Y, Lin YX, Lois C. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2013 May;33:7928-40. [Full Text Article] [PubMed:23637184] [PMC:PMC3853377] Related Antibodies: LS-C60076.

☰ Filters
Antibodies (20)
Primary (20)
RFP / Red Fluorescent Protein (20)
All species (1)
Discosoma (13)
IHC (3)
IHC-Fr (1)
IHC-P (1)
WB (18)
Flo (3)
ELISA (8)
FLISA (1)
ICC (1)
IF (6)
IP (5)
rabbit (5)
mouse (6)
goat (1)
chicken (8)
IgG (8)
IgG1 (1)
IgG1,k (3)
IgG2a,k (2)
IgY (6)
monoclonal mc (6)
polyclonal pc (14)
3G5 (2)
8D6 (1)
8E5.G7 (2)
Agarose Conjugated (1)
APC Conjugated (1)
Biotin Conjugated (3)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (10)
N-Terminus (1)
No (19)
Yes (1)
RFP / Red Fluorescent Protein Antibody - Immunofluorescence Microscopy of Rabbit Anti-RFP antibody. Tissue: HopERCre/+; R26Tom/+ mice. Fixation: 0.5% PFA. Antigen retrieval: Tamoxifen. Primary antibody: RFP antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: Hop-derived cells in the hair follicle, labeled in red.
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RFP / Red Fluorescent Protein Rabbit anti-Discosoma Polyclonal Antibody
Discosoma
ELISA, Flo, IF, IHC, WB
Unconjugated
100 µg/$435
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RFP / Red Fluorescent Protein Goat Polyclonal Antibody
All species
IF, IHC-Fr, IHC-P, WB
Unconjugated
300 µg/$422
Select
RFP / Red Fluorescent Protein Mouse anti-Discosoma Monoclonal (N-Terminus) Antibody
Discosoma
ELISA, IP, WB
Unconjugated
100 µg/$545
RFP / Red Fluorescent Protein Antibody
Select
RFP / Red Fluorescent Protein Mouse Monoclonal (8D6) Antibody
ICC, WB
Unconjugated
100 µl/$451
Select
RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal Antibody
Discosoma
WB
Unconjugated
100 µg/$854
Select
RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (HRP) Antibody
Discosoma
WB
HRP Conjugated
100 µl/$1,073
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RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (Biotin) Antibody
Discosoma
WB
Biotin Conjugated
100 µl/$1,073
Select
RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (FITC) Antibody
Discosoma
WB
FITC Conjugated
100 µl/$1,073
Select
RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (APC) Antibody
Discosoma
WB
APC Conjugated
100 µl/$1,073
Select
RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (PE) Antibody
Discosoma
WB
PE Conjugated
100 µl/$1,073
RFP / Red Fluorescent Protein Antibody - Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. polyclonal anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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RFP / Red Fluorescent Protein Rabbit anti-Discosoma Polyclonal (FITC) Antibody
Discosoma
FLISA, IF, WB
FITC Conjugated
100 µg/$547
RFP / Red Fluorescent Protein Antibody - Western blot of Anti-RFP Antibody. 0.1 ug of RFP was loaded on a 4-20% gel and transferred to nitrocellulose membrane. Anti-RFP Antibody was added at 1.0 g/mL at RT for 2 hours. IRDye800 anti-Chicken (p/n 603-132-126) was added at 1:20000 at RT for 45 minutes.
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RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal Antibody
Discosoma
ELISA, WB
Unconjugated
100 µg/$420
RFP / Red Fluorescent Protein Antibody - Anti-RFP Antibody - Western Blot Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. Separation was achieved using a 4-12% Bis-Tris gradient gel followed by transfer to nitrocellulose and blocking. The membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Goat-a-Rabbit IgG [H&L] MX (611-132-122). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RFP / Red Fluorescent Protein Rabbit anti-Discosoma Polyclonal (Biotin) Antibody
Discosoma
ELISA, IHC, WB
Biotin Conjugated
100 µg/$547
RFP / Red Fluorescent Protein Antibody - Immunofluorescence Microscopy of Chicken Anti-RFP antibody. Tissue: DsRed transgenic mouse retina. Fixation: 4% PFA.3% BSA, 0.3% Triton Primary antibody: RFP-biotin antibody at 1:100 for 12 h at 4°C. Secondary antibody: Alexa488 secondary antibody at 1:10,000 for 4 hours at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: labeled in red.
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RFP / Red Fluorescent Protein Chicken anti-Discosoma Polyclonal (Biotin) Antibody
Discosoma
ELISA, IF, WB
Biotin Conjugated
100 µg/$567
RFP / Red Fluorescent Protein Antibody - Western Blot of Mouse Anti-RFP antibody. Lane 1: YFP protein. Lane 2: Prestained Molecular Weight Marker. Lane 3: Reduced RFP control Protein. Lane 4: Reduced mCherry. Lane 5: GFP protein. Lane 6: Non-Reduced RFP control Protein. Lane 7: Non-Reduced mCherry. Load: 300ng per lane. Primary antibody: RFP antibody at 1:2000 in  Secondary antibody: HRP anti-Mouse secondary antibody at 1:10,000
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RFP / Red Fluorescent Protein Mouse Monoclonal (8E5.G7) Antibody
ELISA, Flo, IF, IP, WB
Unconjugated
100 µg/$574
RFP / Red Fluorescent Protein Antibody - Western Blot of Mouse Anti-RFP antibody. Lane 1: YFP protein. Lane 2: Prestained Molecular Weight Marker. Lane 3: Reduced RFP control Protein. Lane 4: Reduced mCherry. Lane 5: GFP protein. Lane 6: Non-Reduced RFP control Protein. Lane 7: Non-Reduced mCherry. Load: 300ng per lane. Primary antibody: RFP antibody at 1:2000 in MB-070 for 3 hours at RT. Secondary antibody: HRP anti-Mouse secondary antibody at 1:10,000 in MB-070 for 60 min at RT. Substrate: TMBM-100 for 20 min. Predicted/Observed size: ~27 kDa.
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RFP / Red Fluorescent Protein Mouse Monoclonal (8E5.G7) Antibody
ELISA, Flo, IF, IP, WB
Unconjugated
25 µl/$304
RFP / Red Fluorescent Protein Antibody - Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. polyclonal anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Select
RFP / Red Fluorescent Protein Rabbit anti-Discosoma Polyclonal (HRP) Antibody
Discosoma
ELISA, IHC, WB
HRP Conjugated
100 µg/$547
Select
RFP / Red Fluorescent Protein Rabbit Polyclonal Antibody
WB
Unconjugated
50 µg/$449
RFP / Red Fluorescent Protein Antibody
Select
RFP / Red Fluorescent Protein Mouse Monoclonal (3G5) Antibody
IP
Unconjugated
100 µg/$373
RFP / Red Fluorescent Protein Antibody -  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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RFP / Red Fluorescent Protein Mouse Monoclonal (Agarose) (3G5) Antibody
IP
Agarose Conjugated
100 µg/$392
Viewing 1-20 of 20 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).