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Anti-RFP / Red Fluorescent Protein Antibody (HRP) LS-C154272


Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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Rabbit Polyclonal (IgG) to Discosoma RFP / Red Fluorescent Protein
IHC, Western blot, ELISA
HRP Conjugated


Discosoma RFP / Red Fluorescent Protein
Discosoma (tested or 100% immunogen sequence identity)
IgG Polyclonal
Horseradish Peroxidase
Affinity purified


  • IHC (1:500 - 1:2000)
  • Western blot (1:2000 - 1:5000)

Specificity and Use

The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About RFP / Red Fluorescent Protein

Red fluorescent protein (RFP) was originally isolated from Discosoma coral as a tetramer referred to as dsRed. Several synthetic variants of the protein have been created, including monomers. It is commonly used to examine intercellular processes such as tracking gene expression and tagging other proteins.

Requested From: United States
Date Requested: 10/21/2016

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