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Anti-RFP / Red Fluorescent Protein Antibody LS-C60076


Wt. Vol. Conc. Price
100 µg - 1.1 mg/ml Unavailable

Most Popular RFP / Red Fluorescent Protein Antibodies

Anti-RFP / Red Fluorescent Protein Antibody LS-C60076
Rabbit Polyclonal to Discosoma RFP / Red Fluorescent Protein
IHC, Western blot, ELISA
Western blot Image
Anti-RFP / Red Fluorescent Protein Antibody LS-C153868
Chicken Polyclonal (IgG) to Discosoma RFP / Red Fluorescent Protein
Western blot, ELISA
Western blot Image
Anti-RFP / Red Fluorescent Protein Antibody (FITC) LS-C154267
Rabbit Polyclonal (IgG) to Discosoma RFP / Red Fluorescent Protein
Immunofluorescence, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Discosoma RFP / Red Fluorescent Protein
IHC, Western blot, ELISA


Discosoma RFP / Red Fluorescent Protein
Discosoma (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:700000)

Specificity and Use

Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

Publications (1)

Increased cell-intrinsic excitability induces synaptic changes in new neurons in the adult dentate gyrus that require Npas4. Sim S, Antolin S, Lin CW, Lin Y, Lin YX, Lois C. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2013 May;33:7928-40. [Full Text Article] [PubMed:23637184] [PMC:PMC3853377]

About RFP / Red Fluorescent Protein

Red fluorescent protein (RFP) was originally isolated from Discosoma coral as a tetramer referred to as dsRed. Several synthetic variants of the protein have been created, including monomers. It is commonly used to examine intercellular processes such as tracking gene expression and tagging other proteins.

Western blot

Western blot
Anti-RFP Antibody - Western Blot. Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein as a 27 kD band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2500 for 1 h at RT followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Goat-a-Rabbit IgG [H&L] MX (. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: 
Date Requested: 3/26/2017

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