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Antibodies > RFC4 > LS-C482454
 
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Catalog Number Size Price
LS-C482454-50 50 µl (1 mg/ml) $286 
LS-C482454-100 100 µl (1 mg/ml) $321 
LS-C482454-200 200 µl (1 mg/ml) $386 
RFC4 Antibody - Immunohistochemical analysis of RFC4 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
RFC4 Antibody - Immunofluorescent analysis of RFC4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
RFC4 Antibody - Western blot analysis of RFC4 expression in HeLa (A); K562 (B); HepG2 (C); MCF7 (D) whole cell lysates.
RFC4 Antibody - Immunohistochemical analysis of RFC4 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
RFC4 Antibody - Immunofluorescent analysis of RFC4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
RFC4 Antibody - Western blot analysis of RFC4 expression in HeLa (A); K562 (B); HepG2 (C); MCF7 (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human RFC4 Antibody (IHC, IF, WB) LS‑C482454

Polyclonal Rabbit anti‑Human RFC4 Antibody (IHC, IF, WB) LS‑C482454

Submit Review LSBio Guarantee
Antibody:
RFC4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482454-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RFC4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RFC4 antibody LS-C482454 is an unconjugated rabbit polyclonal antibody to RFC4 from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human RFC4
Synonyms
RFC4 | Activator 1 subunit 4 | A1 37 kDa subunit | Activator 1 37 kd subunit | Activator 1 37 kDa subunit | RFC37 | Replication factor C subunit 4 | RF-C 37 kDa subunit | RFC 37 kDa subunit
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length Human RFC4.
Specificity
Recognizes endogenous levels of RFC4 protein.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RFC4
P35249 BC024022 NP_002907.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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