Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits.
Immunoperoxidase of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
RFC4 monoclonal antibody (M01), clone 1C12 Western blot of RFC4 expression in K-562.
Western blot of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RFC4 is approximately 0.3 ng/ml as a capture antibody.
Western blot of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody, clone 1C12. GAPDH ( 36.1 kD ) used as specificity a.
Requested From: United States
Date Requested: 2/21/2017