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Anti-RFC3 Antibody (aa230-258) LS-C162550

Catalog Size Price
LS-C162550-400 400 µl Unavailable

Related Products

18 RFC3 Antibodies

Most Popular RFC3 Antibodies

Anti-RFC3 Antibody (aa80-110) LS-C286647
Rabbit Polyclonal (IgG) to Human RFC3
Human, Mouse, Rat, Bovine, Dog, Xenopus, Zebrafish
IHC, IHC - Paraffin, Immunofluorescence
Unconjugated
Detection of Human RFC3 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RFC3 used at a dilution of 1:250.
Anti-RFC3 Antibody (aa80-110) LS-C287111
Rabbit Polyclonal (IgG) to Human RFC3
Human, Mouse, Rat, Bovine, Dog, Xenopus, Zebrafish
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Detection of Human RFC3 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RFC3 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Anti-RFC3 Antibody (Internal) LS-C352770
Rabbit Polyclonal to Human RFC3
Human, Monkey, Mouse, Rat, Bovine
IHC, IHC - Paraffin, Western blot
Unconjugated
Immunohistochemical analysis of RFC3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-RFC3 Antibody (aa178-227) IHC-plus™ LS-B12568
Rabbit Polyclonal (IgG) to Human RFC3
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-RFC3 Antibody (Internal) LS-C385831
Rabbit Polyclonal (IgG) to Human RFC3
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Western blot of RFC3 antibody

100% Guaranteed
Rabbit Polyclonal to Human RFC3
Human, Mouse
Western blot, Flow Cytometry
Unconjugated

Details

Human RFC3
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Cow (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E7159 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C162550. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa230-258
This RFC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the C-terminal region of human RFC3.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About RFC3

P40938 NM_002915 NP_002906.1

RFC3 Antibody, Activator 1 38 kDa subunit Antibody, A1 38 kDa subunit Antibody, Activator 1 subunit 3 Antibody, Replication factor C subunit 3 Antibody, RF-C 38 kDa subunit Antibody, RFC, 38 kD subunit Antibody, RFC38 Antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.

Western blot

Western blot of RFC3 Antibody in mouse kidney tissue lysates (35 ug/lane). RFC3 (arrow) was detected using the purified antibody.
Western blot of RFC3 Antibody in mouse kidney tissue lysates (35 ug/lane). RFC3 (arrow) was detected using the purified antibody.

Flow Cytometry

RFC3 Antibody flow cytometry of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RFC3 Antibody flow cytometry of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 5/21/2018

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