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Anti-RFC2 / RFC40 Antibody (clone 11C6) LS-C174085

Catalog Size Price
LS-C174085-100 100 µl (1 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 11C6] (IgG1) to Human RFC2 / RFC40
Human
Western blot, Flow Cytometry
Unconjugated

Details

Human RFC2 / RFC40
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [11C6]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

RFC2 / RFC40 antibody was raised against full length human recombinant protein of human RFC2(NP_002905) produced in E. coli.
Human RFC2 / RFC40

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About RFC2 / RFC40

P35250 NP_002905.2

RFC2 Antibody, A1 40 kDa subunit Antibody, Activator 1 40 kDa subunit Antibody, Activator 1 subunit 2 Antibody, Replication factor C 2 Antibody, Rfc40 Antibody, Replication factor C subunit 2 Antibody, RF-C 40 kDa subunit Antibody

RFC2 / RFC40 is a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC2.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RFC2 antibody, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 8/22/2017

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