Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (1:1000 - 1:2000)
Specificity and Use
RFC1 / RFC antibody was raised against a 33 amino acid synthetic peptide which represents a portion of human Replication Factor C 1, 140 kD subunit encoded in part by exons 25 and 26 (LocusLink ID 5981).
Reacts with human RFC1. Other species have not been tested. Probability of reacting with mouse RFC1 is good (25/33 aa are identical, 6/33 aa are conserved substitutions and 2/33 are non-conserved substitutions with the peptide used for immunization).
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000-1:2000.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.