Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > RFC1 / RFC > LS-C312865
 
Print Page  
Catalog Number Size Price
LS-C312865-10 10 µg $318 
LS-C312865-100 100 µg (0.5 mg/ml) $470 
RFC1 / RFC Antibody - IHC analysis of RFC1 using anti-RFC1 antibody. RFC1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-RFC1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
RFC1 / RFC Antibody - WB of RFC1 / RFC antibody. Lane 1: HELA Cell Lysate. Lane 2: SMMC Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: CEM Cell Lysate. Lane 5: 293T Cell Lysate.
RFC1 / RFC Antibody - Western blot analysis of RFC1 using anti-RFC1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Cell Lysate, Lane 2: SMMC Cell Lysate, Lane 3: JURKAT Cell Lysate, Lane 4: CCRF-CEM Cell Lysate, Lane 5: 293T Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150KD. The expected band size for RFC1 is at 128KD.
RFC1 / RFC Antibody - IHC analysis of RFC1 using anti-RFC1 antibody. RFC1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-RFC1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
RFC1 / RFC Antibody - WB of RFC1 / RFC antibody. Lane 1: HELA Cell Lysate. Lane 2: SMMC Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: CEM Cell Lysate. Lane 5: 293T Cell Lysate.
RFC1 / RFC Antibody - Western blot analysis of RFC1 using anti-RFC1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Cell Lysate, Lane 2: SMMC Cell Lysate, Lane 3: JURKAT Cell Lysate, Lane 4: CCRF-CEM Cell Lysate, Lane 5: 293T Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150KD. The expected band size for RFC1 is at 128KD.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human RFC1 / RFC Antibody (aa46‑64, IHC, WB) LS‑C312865

Polyclonal Rabbit anti‑Human RFC1 / RFC Antibody (aa46‑64, IHC, WB) LS‑C312865

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C389147
Antibody:
RFC1 / RFC Rabbit anti-Human Polyclonal (aa46-64) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312865-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RFC1 / RFC Rabbit anti-Human Polyclonal (aa46-64) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
RFC antibody LS-C312865 is an unconjugated rabbit polyclonal antibody to RFC (RFC1) (aa46-64) from human. It is reactive with human and monkey. Validated for IHC and WB.
Target
Human RFC1 / RFC
Synonyms
RFC1 | A1 140 kDa subunit | Activator 1 large subunit | Activator 1 140 kDa subunit | Activator 1 subunit 1 | DNA-binding protein PO-GA | Replication factor C subunit 1 | RF-C 140 kDa subunit | MHC binding factor, beta | PO-GA | MHCBFB | RECC1 | Replication factor C1 | RFC | RFC140
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human RFC1(46-64 aa NSSRKEDDFKQKQPSKKKR).
Epitope
aa46-64
Specificity
Wide tissue distribution. Undetectable in placental tissue.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RFC1 / RFC
P35251 NM_002913 NP_002904.3

Publications (0)

Submit a Publication for LS-C312865

Customer Reviews (0)

Submit a review for LS-C312865

Featured Products

RFC1 / RFC Antibody - Immunofluorescence analysis of A549 cells using RFC1 antibody.
RFC1 / RFC Antibody LS‑C331592
Species: Human
Applications: Immunofluorescence, Western blot
RFC1 / RFC Antibody - Detection limit for recombinant GST tagged RFC1 is approximately 0.3 ng/ml as a capture antibody.
RFC1 / RFC Antibody (clone 3F8) LS‑C198080
Species: Human
Applications: Western blot, ELISA
ACTB / Beta Actin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
IHC‑plus™ ACTB / Beta Actin Antibody (N‑Terminus) LS‑B15553
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IL2RA / CD25 Antibody - Anti-CD25 antibody IHC of human plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ IL2RA / CD25 Antibody LS‑B7396
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Product
KRT8 / CK8 / Cytokeratin 8 Antibody LS‑C193787
Species: Human, Monkey, Bovine, Guinea pig, Hamster, Pig
Applications: IHC, Western blot
Product
ATP1A1 Antibody LS‑C20809
Species: Rabbit, Human, Monkey, Mouse, Rat, Dog, Pig, Xenopus
Applications: IHC, Western blot, Immunoprecipitation
See all RFC1 / RFC Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com