Mouse Retnla / RELM Alpha
(tested or 100% immunogen sequence identity)
- Western blot (1 - 5 µg/ml)
- ELISA (1:10000 - 1:100000)
A 14-aa peptide sequence near the N-terminus of mouse RELM-alpha (1) was coupled with KLH (carrier protein).
Mouse sequence is only 50% conserved in rat and no appreciable homology with human RELM-alpha. We recommend the use of antibody that is raised to human RELM-alpha sequence. No significant sequence was found with resistin or RELM-beta. Antibody cross-reactivity in various species is not established. Control peptides, because of their small size (< 2kD), are not suitable for Western. We recommend the use of control peptide in antibody blocking experiments (use 5-10 ug of peptide per 1 ug of aff pure or 1 ul of antiserum) to demonstrate antibody specificity.
Suitable for use in ELISA, Western Blot. Western Blot: 1-5 ug/ml ECL. Resistin is ~9kD. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.
PBS, pH 7.4, 0.1% BSA.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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