Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG2a) to Rat Retnla / RELM Alpha
Western blot, ELISA
Rat Retnla / RELM Alpha
Rat (tested or 100% immunogen sequence identity)
Western blot (1:5000 - 1:10000)
Specificity and Use
Recombinant His-tagged rat RELM- (resistin-like molecule- ) produced in E. coli.
Recognizes rat RELM-. Detects a band of ~10.5kD by Western blot. Does not cross-react with mouse RELM- or human resistin.
Suitable for use in ELISA, Western Blot. ELISA (direct and indirect): 1:5000-1:10000). Western Blot: 1:5000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at RT).
Resistin-like molecule alpha (Retnla, also known as "Found in inflammatory zone 1" (Fizz1) is a hypoxia-induced mitogenic factor. It is highly induced in lung allergic inflammation and lung fibrosis, and primarily expressed by airway and type II alveolar epithelial cells. It stimulates alpha-smooth muscle actin and collagen expression in lung fibroblasts.