Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal (IgG2a) to Mouse Retnla / RELM Alpha
Western blot, ELISA
Mouse Retnla / RELM Alpha
Mouse (tested or 100% immunogen sequence identity)
Specificity and Use
Recombinant mouse His-tagged RELM- (resistin-like molecule- ) expressed in E. coli.
Recognizes mouse RELM-. Detects a band of ~10kD by Western blot.
ELISA (direct and indirect: 1:5000-1:10000). Western Blot (1:5000-1:10000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Resistin-like molecule alpha (Retnla, also known as "Found in inflammatory zone 1" (Fizz1) is a hypoxia-induced mitogenic factor. It is highly induced in lung allergic inflammation and lung fibrosis, and primarily expressed by airway and type II alveolar epithelial cells. It stimulates alpha-smooth muscle actin and collagen expression in lung fibroblasts.