LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-RET Antibody LS-C75422

Ordering

Wt. Vol. Conc. Price
500 µg 1000 µl 0.5 mg/ml $635
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RET Antibodies

Anti-RET Antibody (aa13-44) LS-C99120
Rabbit Polyclonal to Human RET
Human
Western blot, Flow Cytometry
Unconjugated
Western blot Image
Anti-RET Antibody (aa896-1063, clone 8D10C9) IHC-plus™ LS-B6328
Mouse Monoclonal [clone 8D10C9] (IgG1) to Human RET
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RET Antibody (aa1012-1042) IHC-plus™ LS-B6923
Rabbit Polyclonal to Human RET
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human RET
Human, Mouse
Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human RET
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry (50 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

RET antibody was raised against recombinant protein corresponding to aa29-635 from human Ret/Fc Chimera expressed in Sf21 cells (P07949).
Recognizes human Ret. Species cross-reactivity: Mouse.
Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 ug/ml; For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Western Blot: 1-2 ug/ml; Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 ug/ml; The detection limit for rhRet and rmRet is ~6 ng/well.

Packaging

Lyophilized from PBS, 5% trehalose.
1,000 µl Sterile PBS
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.

About RET

P07949 NM_020630 NP_065681.1

RET Antibody, Cadherin family member 12 Antibody, CDHF12 Antibody, C-ret Antibody, Hirschsprung disease 1 Antibody, HSCR1 Antibody, Hydroxyaryl-protein kinase Antibody, Oncogene ret Antibody, Proto-oncogene c-Ret Antibody, RET-ELE1 Antibody, RET51 Antibody, Ret proto-oncogene Antibody, RET transforming sequence Antibody, RET43 Antibody, Men2-ret Antibody, MEN2B Antibody, MTC1 Antibody, Receptor tyrosine kinase Antibody, CDHR16 Antibody, MEN2A Antibody, PTC Antibody, RET9 Antibody

Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information.

Flow Cytometry

Flow Cytometry
Flow Cytometry Figure - Cell Count vs. Ret: SHSY-5Y human neuroblastoma cells were stained with LS-C75422 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.
 

Requested From: United States
Date Requested: 12/10/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number