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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Requiem / DPF2 Antibody (aa151‑200) LS‑C119185
DPF2 antibody LS-C119185 is an unconjugated rabbit polyclonal antibody to DPF2 (Requiem) from human and mouse. Validated for IF, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C119185-50
50 µl
Unavailable
LS-C119185-100
100 µl
Unavailable

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Immunohistochemical analysis of DPF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Product Description

DPF2 antibody LS-C119185 is an unconjugated rabbit polyclonal antibody to DPF2 (Requiem) from human and mouse. Validated for IF, Peptide-ELISA and WB.
About Requiem / DPF2
Requiem / DPF2 is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Q92785 NM_006268 NP_006259.1

DPF2 Antibody, BAF45D Antibody, REQ Antibody, UBID4 Antibody, Zinc finger protein ubi-d4 Antibody, BRG1-associated factor 45D Antibody, Ubi-d4 Antibody, Protein requiem Antibody, Requiem Antibody


Specifications

Target
Human Requiem / DPF2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Immunogen
Requiem / DPF2 antibody was raised against synthetic peptide from human REQU (aa151-200).
Epitope
aa151-200
Specificity
REQU Antibody detects endogenous levels of total REQU protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence

Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence

Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence

Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of A549 cells, using REQU Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from HT-29 cells, using REQU Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/18/2018

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