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Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652

Note: This antibody replaces LS-C18901, LS-C9298

Ordering

Wt. Vol. Conc. Price
- 50 µl 75 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular RELA / NFKB p65 Antibodies

Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (aa526-539, clone 112A1021) IHC-plus™ LS-B6438
Mouse Monoclonal [clone 112A1021] (IgG1,k) to Human RELA / NFKB p65
Human, Monkey, Mouse, Bovine, Dog, Pig
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (4)
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human RELA / NFKB p65
Rabbit
Human, Monkey, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

pSer529
NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: PNGLLpSGDEDFC.
Immunohistochemistry: LS-B652 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B652 was determined to be 1:500.

Packaging

Serum, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (4)

1
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB. Journal of immunology (Baltimore, Md. : 1950). 2002 169:6490-7. [PubMed:12444159]
2
The phosphatidylinositol 3-kinase/Akt pathway negatively regulates Nod2-mediated NF-kappaB pathway. Zhao L, Lee JY, Hwang DH. Biochemical pharmacology. 2008 75:1515-25. [PubMed:18243161]
3
Role of NF-kappaB in flow-induced vascular remodeling. Castier Y, Ramkhelawon B, Riou S, Tedgui A, Lehoux S. Antioxidants & redox signaling. 2009 11:1641-9. [PubMed:19320561]
4
Anti-inflammatory and anti-catabolic effects of TENDOACTIVE on human tenocytes in vitro. Shakibaei M, Buhrmann C, Mobasheri A. Histology and histopathology. 2011 26:1173-85. [PubMed:21751149]
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About RELA / NFKB p65

Q04206 NM_021975 NP_068810.3

RELA Antibody, NF-kappa-B p65delta3 Antibody, NFKB3 Antibody, p65 Antibody, Transcription factor p65 Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B652 dilution 1:500.

Immunohistochemistry

Immunohistochemistry
Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B652 dilution 1:500.

Western blot

Western blot
Anti-pS529 shows phospho p65 staining in carcinoma cells.

Western blot

Western blot
Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. pan reactive anti-p65 (code# LS-B653) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the Western blot of a chemiluminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB.

Western blot

Western blot
Top row is anti-pan p65. Bottom row is anti-pS529 showing phospho p65 staining in carcinoma cells. Immunoblot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and

Western blot

Western blot
Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells.

Requested From: United States
Date Requested: 12/7/2016

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