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Anti-RELA / NFKB p65 Antibody (phospho-Ser276) LS-C18906

Ordering

Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RELA / NFKB p65 Antibodies

Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (aa526-539, clone 112A1021) IHC-plus™ LS-B6438
Mouse Monoclonal [clone 112A1021] (IgG1,k) to Human RELA / NFKB p65
Human, Monkey, Mouse, Bovine, Dog, Pig
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human RELA / NFKB p65
Human
Western blot, ELISA
Unconjugated

Details

Human RELA / NFKB p65
Rabbit
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA (1:10000 - 1:30000)

Specificity and Use

pSer276
NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: QLRRPpSDRELSC.
This phospho specific polyclonal antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. A 65 kD band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control.

Packaging

Store vial at -20 C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below.
For research use only.

About RELA / NFKB p65

Q04206 NM_021975 NP_068810.3

RELA Antibody, NF-kappa-B p65delta3 Antibody, NFKB3 Antibody, p65 Antibody, Transcription factor p65 Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Western blot

Western blot
Western Blot - Anti-NFKB p65 (Rel A) pS276 Antibody. TNF Induces phosphorylation of p65 in KBM-5 cells.

Western blot

Western blot
Western Blot - Anti-NFKB p65 (Rel A) pS276 Antibody. TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. pan reactive anti-p65 (code# LS-B653) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB.

Western blot

Western blot
Western Blot - Anti-NFKB p65 (Rel A) pS276 Antibody. Anti-pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamous carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamous carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Other detection methods will yield similar results. Data contributed by Yu, M., NIH, personal communication.

Western blot

Western blot
Top row is anti-pan p65. Bottom row is anti-pS276 showing phospho p65 staining in carcinoma cells. Immunoblot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 con

Western blot

Western blot
Western Blot - Anti-NFKB p65 (Rel A) pS276 Antibody. Anti-pS529 shows phospho p65 staining in carcinoma cells.
 

Requested From: United States
Date Requested: 12/10/2016

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