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Anti-RELA / NFKB p65 Antibody (N-Terminus) LS-C18905

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular RELA / NFKB p65 Antibodies

Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (aa526-539, clone 112A1021) IHC-plus™ LS-B6438
Mouse Monoclonal [clone 112A1021] (IgG1,k) to Human RELA / NFKB p65
Human, Monkey, Mouse, Bovine, Dog, Pig
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (3)
Rabbit Polyclonal to Human RELA / NFKB p65
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human RELA / NFKB p65
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200)
  • Western blot (1:2000)
  • ELISA

Specificity and Use

N-Terminus
The NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Suitable for immunohistochemistry, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against NFxB p65 NLS at a dilution of 1:2000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NFxB NLS is suitable for the gel supershift assay and was found to be reactive against all p65 (Rel A) containing human NFxB complexes using 0.5 to 1.0 ul per assay.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

Publications (3)

1
Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex. Starkey JM, Haidacher SJ, LeJeune WS, Zhang X, Tieu BC, Choudhary S, Brasier AR, Denner LA, Tilton RG. Diabetes. 2006 55:1252-9. [PubMed:16644679]
2
Antiinflammatory adaptation to hypoxia through adenosine-mediated cullin-1 deneddylation. Khoury J, Ibla JC, Neish AS, Colgan SP. The Journal of clinical investigation. 2007 117:703-11. [PubMed:17318263] [PMC:PMC1797604]
3
Adenosine signaling mediates SUMO-1 modification of IkappaBalpha during hypoxia and reoxygenation. Liu Q, Li J, Khoury J, Colgan SP, Ibla JC. The Journal of biological chemistry. 2009 284:13686-95. [PubMed:19297320] [PMC:PMC2679470]

About RELA / NFKB p65

Q04206 NM_021975 NP_068810.3

RELA Antibody, NF-kappa-B p65delta3 Antibody, NFKB3 Antibody, p65 Antibody, Transcription factor p65 Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-p65 NLS Antibody - Immunohistochemistry. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) control DU145 cells and (B) TNF stimulated DU145 cells. Although DU145 show relatively high basal levels of nuclear p65 staining, significant enhancement of nuclear staining is seen in panel B as evidence of translocation and availability of the NLS to be bound by the antibody. Cultured cells shown above were formalin-fixed. tissue staining (not shown) were formalin-fixed, paraffin embedded followed by citrate retrieval. Blocking and hybridization included 5% NGS.

Immunofluorescence

Immunofluorescence
Anti-p65 NLS Antibody - Immunofluorescence Microscopy. Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in TNF stimulated DU145 cells. Image shown is at a 1:400 magnification. Tissue was fixed and prepared as above.

Requested From: United States
Date Requested: 12/5/2016

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