Human RELA / NFKB p65

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated

Unmodified

NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

C-Terminus

• Western blot (1:500 - 1:3000)
• ELISA (1:5000 - 1:25000)
• Gel shift (1:500)

Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against NFxB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF?B cRel is suitable for the detection by immunoblot of human NF?B cRel. No reaction was observed against the analogous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NF?B complexes using 0.5 to 1.0 ?l per assay. Optimal titers for other applications should be determined by the researcher.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q04206 NCBI: NM_021975 NP_068810.3