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Anti-RELA / NFKB p65 Antibody (C-Terminus) IHC-plus™ LS-B653

Note: This antibody replaces LS-C18902

Ordering

Wt. Vol. Conc. Price
- 50 µl 80 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RELA / NFKB p65 Antibodies

Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus™ LS-B652
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Monkey, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RELA / NFKB p65 Antibody (aa526-539, clone 112A1021) IHC-plus™ LS-B6438
Mouse Monoclonal [clone 112A1021] (IgG1,k) to Human RELA / NFKB p65
Human, Monkey, Mouse, Bovine, Dog, Pig
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (9)
Rabbit Polyclonal to Human RELA / NFKB p65
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA, Gel shift
Unconjugated

Details

Human RELA / NFKB p65
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Gel shift

Specificity and Use

C-Terminus
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunohistochemistry: LS-B653 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B653 was determined to be 1:500.

Packaging

0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (9)

1
Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo. Unlap MT, Jope RS. Brain research. Molecular brain research. 1997 45:83-9. [PubMed:9105673]
2
Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity. Jobin C, Bradham CA, Russo MP, Juma B, Narula AS, Brenner DA, Sartor RB. Journal of immunology (Baltimore, Md. : 1950). 1999 163:3474-83. [PubMed:10477620]
3
Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype. Poser I, Golob M, Buettner R, Bosserhoff AK. Molecular and cellular biology. 2003 23:2991-8. [PubMed:12665595] [PMC:PMC152547]
4
NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. Cao S, Zhang X, Edwards JP, Mosser DM. The Journal of biological chemistry. 2006 281:26041-50. [PubMed:16835236] [PMC:PMC2642587]
5
Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms. Lou H, Kaplowitz N. The Journal of biological chemistry. 2007 282:29470-81. [PubMed:17690092]
6
CXCL5 promotes prostate cancer progression. Begley LA, Kasina S, Mehra R, Adsule S, Admon AJ, Lonigro RJ, Chinnaiyan AM, Macoska JA. Neoplasia (New York, N.Y.). 2008 10:244-54. [PubMed:18320069] [PMC:PMC2262133]
7
Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. Yoon C, Korade Z, Carter BD. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2008 28:3738-46. [PubMed:18385332]
8
Axonal neuregulin 1 type III activates NF-kappaB in Schwann cells during myelin formation. Limpert AS, Carter BD. The Journal of biological chemistry. 2010 285:16614-22. [PubMed:20360002] [PMC:PMC2878070]
9
Nuclear factor-B contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes. Ghosh M, Yang Y, Rothstein JD, Robinson MB. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:9159-69. [PubMed:21697367] [PMC:PMC3138498]
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About RELA / NFKB p65

Q04206 NM_021975 NP_068810.3

RELA Antibody, NF-kappa-B p65delta3 Antibody, NFKB3 Antibody, p65 Antibody, Transcription factor p65 Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B653 dilution 1:500.

Immunohistochemistry

Immunohistochemistry
Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B653 dilution 1:500.

Western blot

Western blot
Anti-NFkb p 65 Rel A Antibody - Western Blot. Western blot of HeLa cell extract. A predominant band ~65 kD (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.

Western blot

Western blot
Immunoblot of HeLa cell extract using anti-NFkb p65 (RelA) at a 1:5000 dilution. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed

Requested From: United States
Date Requested: 12/3/2016

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